January 2005: PhyNexus and Caliper Life Sciences give joint Poster Presentation at CHI's PEP Talk.
PhyNexus presented a joint poster with Caliper Life Sciences at the recent PEP talk titled, "Improved process efficiency with 96-well protein purification and characterization." In this poster, PhyNexus presented an integrated approach that included the automated purification of micro volume protein samples in a 96-well format along with real time monitoring of the resulting purity and concentration data.
The research focused on the simultaneous purification and enrichment of up to 96 samples in under 15 minutes. This was performed by automated selective enrichment of monoclonal antibodies from hybridoma growth media and recombinant 6X-His or GST-tagged fusion proteins from E. coli lysate using PhyTip columns on the Sciclone ALH 3000 workstation. Subsequent characterization (size, concentration, purity) of the enriched material using the LabChip 90 microfluidic protein analysis system provides a streamlined workflow and significant reduction in time and labor associated with high throughput protein expression and purification. The purity and concentration of the final products enable downstream analytical and biochemical assays, increasing productivity and decreasing development time for therapeutic antibody drugs.
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