December 2004: PhyNexus presents joint poster with Caliper Life Sciences at IBC's 15th annual international conference on Antibody Engineering San Diego, CA.
PhyNexus presented a poster titled: Improved process efficiency with 96-well protein purification and characterization, a joint presentation with Caliper Life Sciences that detailed the use of the Caliper LH 3000, PhyTip columns and Caliper LC 90 detection system for high throughput purification, enrichment and detection of Antibodies and recombinant proteins.
During the scientific session PhyTip products were featured twice in talks by Dr Dev Sidhu of Genentech and Dr Clive wood of Dyax:
Dr Dev Sidhu of Genentech discussed his research involving phage-displayed antibody libraries with synthetic complementary determining regions. The purpose of the work was to investigate the basic principals of antigen recognition using libraries containing limited diversity. These libraries could lead to the generation of new antibody reagents and therapeutics.
Dr Sidhu used micro scale PhyTip columns for high throughput purification and enrichment of Fabs enabling him to produce high concentrations of the functional proteins for assay by SPR. This work required the generation of large numbers of samples and the problem of expressing and then purfying these samples was solved by the use of PhyTip technology. PhyTips enabled the high throughput process of rapidly purifying 96 samples at a time, producing high concentrations of functional protein that could be assayed directly, speeding up the whole discovery process.
Dr Clive Wood discussed the process developed at Dyax for Antibody drug discovery which requires a rapid and reliable means to identify high-affinity leads. In combination with automated selection and screening methodologies, thousands of Fab hits can be examined and in multiple projects, this has led to the rapid identification of very potent and selective Fab leads without in vitro affinity maturation. Dr Wood reviewed the use of PhyTip columns in their process of high throughput purification. PhyTips columns were necessary for the parallel processing of 96 samples at a time producing high concentrations of functional Fabs that could be assayed directly with the high throughput SPR system from HTS Biosystems.
|
|