Due to the unique flexibility of design, PhyTip® column technology can be used for a diverse range of applications allowing researchers to implement optimal preparation strategies that maximize the quality and quantity of final product. PhyTip column technology is being used in each of the 5 steps of the drug discovery and development process in most major Biopharma companies worldwide, and by many academic research groups. Due to the complex nature of biological samples, protein analysis techniques require fractionation of the sample prior to analysis and by using PhyTip column technology, researchers have been able to economically replace currently tedious and laborious techniques to:

• Reduce false positives
• Save time and resources
• Make better informed decisions earlier in the process
• Scale the technology - in both sample volume and sample number

With an increasing number of experiments that have successfully used PhyTip column technology, the breadth of application areas has also expanded greatly

PhyTip column uses in the drug discovery and development process

1. Target Discovery and Validation:

Protein Profiling - identification of potential therapeutic targets based on differential protein expression levels.
Protein Interaction - including IP and Co-IP

Pathway Activation & Mapping - determining the protein-protein interactions involved in signaling cascades, characterizing upstream and downstream avenues modulating target activity and response, ChIP, phosphoprotein enrichment.

Target Expression & Purification - expressing an identified target protein and preparing sufficient quantities for further studies including library screening, functional assays and structural analysis.

Biomarker Discovery

Biomarker Validation

2. Drug Screening and Lead Optimization:

Library synthesis Library Screening and Selection Assay Development - producing high quality functional data in less time and requiring fewer resources.

Lead Characterization - preparing higher purity material for affinity maturation, kinetic assays and Antibody specificity, selectivity and affinity.

Lead Optimization - affinity maturation

Structural Analysis - more efficiently allowing for both condition screening and preparing high quality products ready for analysis.

3. Development:

Process Development - screening of purification conditions and faster turnaround times in trial-scale studies.

PhyTip columns and Analytics - monitoring purity and post translational modification status in a reproducible and economical process, oligosaccharide mapping of glycoproteins.

Pre-clinical Assays - purification and immunogenicity studies.

4. Clinical Trials:

Immunogenicity studies - performing acid-dissociation of anti-drug antibodies (ADAs) and drug followed by PhyTip column purification of ADAs allows a more accurate depiction of immunogenicity

5. Manufacturing:

Scale-up - due to the chromatographic nature of the columns, the same optimization conditions and resin can be used for large scale separations, saving resources during optimization.