The unique design of the PhyTip affinity columns allows bidirectional flow of the sample through the column to maximize the use of the resin by increasing capture and elution of the target protein, leading to the highest yield possible. The screens surrounding the resin at the base of the column allow elution volumes as low as 10µL, and therefore high concentration product. In addition, the high efficiency bidirectional washes lead to the highest possible sample purity. Perform affinity purification, ion exchange, desalting or buffer exchange applications.

PhyTip gel filtration columns operate as small scale, chromatographic separation columns. They represent the only high throughput, automatable, chromatography desalting solution. The gel filtration columns are similar to the PhyTip affinity columns in that the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column. Unlike the affinity columns, the sample is passed unidirectionally through the gel filtration columns by gravity flow. The gel filtration columns remove >95% of salts with >80% yield of protein, retain protein functionality, provide less than 10% CV for volume and concentration between final sample and provide functional protein that is ready for the downstream assay.

Researchers often need to settle for the best results obtained from a few purification optimization steps. One of the benefits of a high throughput small-scale purification method is the ability to easily and quickly screen many purification options simultaneously. PhyNexus has made this even easier with the availability of the PhyScreen Ni-IMAC screen kit which includes columns of twelve different Ni-IMAC resins, prepared for ease of use with the MEA automated system, or other commercially available liquid handlers. Resin screening is especially valuable when affinity purifying His-tagged recombinant proteins due to the high propensity of background interactions between Ni-IMAC and naturally occurring multiple His residues.

PhyTip Normal Phase Resin columns are designed for the specific enrichment of polar compounds. These PhyTip columns are especially useful in oligosaccharide mapping and identification analysis of glycoproteins. High resolution tools such as HPLC and capillary electrophoresis are required for the separations of these complicated structures; however, detection must be enhanced through use of fluorescent dye labels. PhyTip Normal Phase Resin columns are the perfect solution for high throughput, reproducible sample dye removal prior to identification and quantification.

Whether separations are performed a few times a week or a few thousand times a day, PhyTip columns may be used with manual pipettes, the ME or MEA purification systems, or other commercially available robotic systems. The PhyTip ME Purification Systems allow for complete flexibility and semi-automated processing of 1-12 samples at a time. The MEA robot fills the productivity gap between the ME semi automated instruments and 96 channel capability robots.