For more technical information about PhyTip Affinity Columns, see the Technology Affinity Columns section. PhyNexus is introducing a new member to the repertoire of standard PhyTip® column products. Combining the proven technology of PhyTip affinity columns with the benefits of a new resin demonstrating attributes of both Protein A and Protein G, results in an antibody purification platform that exhibits superior purification results.
- At least 10% higher capacity than Protein A resins
- Broad range of selectivity, recognizing various isotypes and species, similar to Protein G
- 3-5 fold greater recovery than Protein G (3-fold shown below)
Conclusion
-The table above demonstrates that PhyTip ProPlus columns process samples to capture, purify and enrich 3 fold greater recoveries of protein compared to Protein G columns.
-Protein G resin is valuable due to selectivity for antibody subtype or species. However, Protein G resins have a much lower capacity than Protein A resins resulting in lower recovery of purified antibody.
-ProPlus exhibits an ideal combination of Protein A and Protein G, providing the desired selectivity and capacity, making it the resin of choice for antibody purification.