For more technical information about PhyTip Affinity Columns, see the Technology Affinity Columns section. Protein A PhyTip Columns demonstrate high binding affinity and high capacity affinity purification of functional antibodies (IgGs).
- High binding affinity and high capacity
- 70-90% IgG recovery.
Protein A Product Insert Sheet
Figure 1.
Figure 2.
Figure 2. Reducing SDS-PAGE gel of a murine mAb IgG2a enriched from varying levels of background proteins. The heavy and light chain bands of the pure IgG are shown in lane 2. Lane 3 contains mAb after enrichment from PBS. The remaining lanes show the purified mAb after enrichment from 5 mg/ml BSA (lane 4), DMEM+10% FBS (lane 5), serum-free media with 5 mg/ml BSA. No background proteins are detected in the enriched samples.
Conclusion
-Obtain highly concentrated purified proteins (Fig 1).
-Protein A PhyTip columns are shown to extract IgG proteins with very high purity from varying levels of background proteins (Fig 2 no back ground proteins detected).