For more technical information about PhyTip Affinity Columns, see the Technology Affinity Columns section. Protein G PhyTip Columns demonstrate high binding affinity and high selectivity affinity purification of functional antibodies (IgGs).
- High binding affinity
- High selectivity for different species and isotypes of antibody
- 70-90% IgG recovery.
Protein G Product Insert Sheet
Figure 1.
Figure 2.
Fig 2: Reducing SDS-PAGE gel of a murine mAb IgG2a enriched from samples containing BSA. Lanes 1 and 3 show purified mAb after enrichment from 5 mg/mL BSA. Lanes 2 and 4 show purified mAb after enrichment from PBS buffer. Lane 5 is pure IgG2a showing heavy and light chains.
Conclusion
-Obtain highly concentrated purified proteins (Fig 1).
-Protein G PhyTip columns are shown to have high selectivity for varying isotypes and species of IgG. Shown above murine IgG2a is enriched from various samples.