The unique design of the PhyTip affinity columns allows bidirectional flow of the sample through the column to maximize the use of the resin by increasing capture and elution of the target protein, leading to the highest yield possible. The screens surrounding the resin at the base of the column allow elution volumes as low as 10μL and therefore high concentration product. In addition, the high efficiency bidirectional washes lead to the highest possible sample purity. Perform affinity purification, ion exchange, desalting or buffer exchange applications.

PhyTip gel filtration columns operate as small scale, chromatographic separation columns. They represent the only high throughput, automatable, chromatography desalting solution. The gel filtration columns are similar to the PhyTip affinity columns in that the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column. Unlike the affinity columns, the sample is passed unidirectionally through the gel filtration columns by gravity flow. The gel filtration columns remove >95% of salts with >80% yield of protein, retain protein functionality, provide less than 10% CV for volume and concentration between final sample and provide functional protein that is ready for the downstream assay

Whether separations are performed a few times a week or a few thousand times a day, PhyTip columns may be used with manual pipettes, the ME or MEA purification systems, or other commercially available robotic systems. The PhyTip ME Purification Systems allow for complete flexibility and semi-automated processing of 1-12 samples at a time. The MEA robot fills the productivity gap between the ME semi automated instruments and 96 channel capability robots.