Summary

PhyTip columns are the most efficient and cost effective way to purify recombinant proteins and antibodies using standard affinity resins.

• Their unique design allows bidirectional flow (see arrow in figure) of the sample through the column to maximize the use of the resin by increasing capture and elution of the target protein, leading to the highest yield possible.
• The design of the screens surrounding the resin allows elution volumes as low as 10μL, and therefore high concentration product.
• The high efficiency bidirectional washes lead to the highest possible sample purity.
• Perform affinity purification with our standard resins or any custom resin of your choice.
• Obtain higher performance than with any other resin purification method.

Product Benefits

• Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
• Process small sample volumes in a reproducible, high throughput, automatable format
• Elution volumes as low as 10 μL, producing enrichment factors as high as 50 fold, with concentrations of purified protein of up to 10 mg/mL

Sample Separation

Figure 2. Affinity purification of antibody sample ... Capture, Purify, Enrich

Pane#1. Low concentration Alexa488-labeled antibody showing very little fluorescence.

Pane#2. Protein A PhyTip column after processing antibody sample. Alexa488-labeled antibody has been successfully captured on the PhyTip column. The sample is purified by washing the column.

Pane#3. PhyTip column after the elution step; there is little discernible fluorescence due to the very high yield for the separation.

Pane#4. High enrichment of the Alexa488-labeled antibody is achieved after elution.

Patent pending PhyTip® column technology is a unique walk away bench-top separation process controlled with a high level of precision for life science researchers who prepare protein samples for analysis. By utilizing industry standard resins, it allows for the unattended parallel processing of a range of samples and volumes providing the highest flexibility in meeting the needs of a diversity of applications. Columns can be used to process sample volumes from 10ul to 25ml (sample volumes exceeding the tip column volume are processed as a series of fractions during the capture step).

Each column is manufactured to the highest standards by first ensuring consistent length of each column, followed by welding of the inert screen at the base of the column. The proprietary column manufacturing process ensures that a consistent volume of resin is delivered and packed into the column prior to the placement of a second inert screen at the top of the resin bed. PhyTip columns are then extensively tested to ensure consistency of flow before packing and storage.